Differential effects of high fat diet and dietinduced. Stern, and jules hirsch rockefeller university, new york, new york 10021 faust, irving m. Body fat accumulation in zebrafish is induced by a diet. This book contains proven steps and strategies on how to know about childhood obesity and take necessary step to prevent it. Average weekly feed intake was similar between lean and obese mice. Nicotine plus a highfat diet leads to intramyocellular fat. Read on your pc, mac, smartphone, tablet or kindle device. Background nutritional strategies to treat obesity often influence neuroendocrine factors related to body weight control. Pdf body fat accumulation in zebrafish is induced by a. This dio zebrafish exhibits increased body weight gain, hypertriglyceridaemia and hepatic steatosis.
Protective effects of bl153 on obesityinduced renal. Dietinduced obesity suppresses the secretory capacity of the mgs. To determine whether this also occurs in larvae, zebrafish fed with hf diet and hfc diet were stained with oil red o. Discover a faster, simpler path to publishing in a highquality journal. Leptin is an adipokine involved in body weight and food intake regulation whose promoter region presents cpg islands that could be subject to dynamic methylation. C57bl6ntac males are put on special diet at 6 weeks of age and housed at reduced density. Dec 11, 2017 upregulation of inos has been clearly implicated in obesity and diabetes 10,11, and no overproduction induced by highfat diet was reported to downregulate the insulin signaling in skeletal muscle. Mar 15, 2016 nongenetic inheritance of diet induced obesity in mice epigenetic factors transmitted in mice through the sperm and eggs gametes predispose the offspring of obese parents to diet induced obesity, reports a paper published online this week in nature gene. Control zebrafish maintained their weight during the 8week. The overall gene expression profile of visceral adipose tissue is also consistent with those of humans and rodents. Sugarinduced obesity and insulin resistance are uncoupled.
Obesity but not high fat diet results in lymphatic dysfunction. Pdf body fat accumulation in zebrafish is induced by a diet. Basic nutritional investigation ingested cocoa can prevent. To better understand the pathomechanisms of human disease, good animal models are essential. Highfat dietinduced obesity in animal models niloofar hariri and louise thibault school of dietetics and human nutrition, mcgill university, quebec, canada epidemiological studies have shown a positive relationship between dietary fat intake and obesity. The tonle sap lake cambodia kong heng, mathieu chevalier, sovan lek, pascal laffaille.
Animal models of obesity are required to help us understand the signaling pathways underlying this condition. Obesity is associated with brains neurotransmitters. Researcharticle magnolia extract bl153 ameliorates kidney. Adult zebrafish have also been used as an obesity model. Diet the definitive guide to a plantbased diet in a world where nutrition books are bestsellers and a different supplement is promising the shortcut to health on instagram every week, the word diet is as much a part of our lives as the latest netflix romcom or seasonal fashion trend. The first diet induced obese dio zebrafish model was reported in 2010 oka et al. Oct 21, 2010 although zebrafish larvae have been used in genetic and chemical screening experiments to identify novel genes involved in the regulation of energy homeostasis and potential therapeutic targets to treat obesity,23, it remains unclear whether zebrafish can be used as a model for diet induced obesity dio, similar to that observed in mammals. For this, they used a vital dye, nile red, which is incorporated into adipose tissue during tissue formation 4547. Diet induced obesity models diets high in fat have been attributed to a host of diseases, including obesity, metabolic and inflammatory diseases. Body fat accumulation in zebrafish is induced by a diet rich.
Mar 21, 2017 we assessed dietinduced obesity in zebrafish by overfeeding adult male zebrafish with normalfatdiet nfdof or highfatdiet hfdof compared to physiological control diet for 8 weeks fig. May 20, 2015 diet induced hepatic steatosis is highly associated with nonalcoholic fatty liver disease, which is related to the development of metabolic syndrome. Seasonal variations in diet composition, diet breadth and dietary overlap between three commercially important fish species within a floodpulse system. Therefore, the goal of the current study was to analyze the effects of high fat diet induced obesity hfd on lymphatic function in obese prone and obese resistant mice. Dietinduced obesity in zebrafish shares common pathophysiological pathways with mammalian obesity. This methylation process could be affected by environmental e. In fact, diet induced obesity and age are major risk factors for developing t2d, yet despite clear correlations between insulin resistance, obesity, and aging, the exact underlying mechanisms still remain unclear chatterjee et al. Obesity amplifies genetic risk of nonalcoholic fatty liver. Moreover, a useful zebrafish model of dietinduced obesity dio has been created and was found to be highly consistent with the obesity observed in humans and in rodent models of dio oka et al. We hypothesized that the combination of high saturated fat diet, obesity, and. The jackson laboratory maintains an inventory of diet induced obesity dio models, and can place any strain on any fully customized diet so that your mice are ready when you need them. Impact of physician bmi on obesity care and beliefs. Hf or hfc diets exposure results in steatosis and hepatitis in adult fish and rodents models 25, 35.
We determined the anti obesity effects of boiled tuna extract 100, 200, or 400 mgkg on the progression of hfd induced obesity for 10 weeks. Body fat accumulation in zebrafish is induced by a diet rich in fat and reduced by supplementation with green tea extract article pdf available in plos one 103. C57bl6ntac males are put on special diet at 6 weeks of age and. One group of zebrafish was overfed with artemia 60 mg dry weightday fish, a living prey consisting of a relatively high amount of fat. Effects of yuzu citrus junos siebold ex tanaka peel on. We optimized protocols for generating dietinduced obese dio zebrafish model by feeding high cholesterol diet hcd. A new basicscience study showing that cholesterol supplementation prevents fetal alcohol defects in zebra fish embryos has reignited debate about whether there is. Rhamnan sulphate from monostroma nitidum attenuates. Antiobesity effects of boiled tuna extract in mice with. Nongenetic inheritance of dietinduced obesity in mice epigenetic factors transmitted in mice through the sperm and eggs gametes predispose the offspring of obese parents to dietinduced obesity, reports a paper published online this week in nature gene. Prevalence of obesity and trends in the distribution of body mass index among us adults, 19992010. Both, nfdof and hfdof fish had an enlarged belly compared to control fish fig. Obesity significantly amplifies the effects of three gene variants that increase risk of nonalcoholic fatty liver. Apr 16, 2020 obesity is a substantial public health crisis in the united states and in the rest of the industrialized world.
A randomised study on the effects of fish protein supplement on glucose tolerance, lipids and body composition in overweight adults volume 109 issue 4 linn a. Skeletal muscle contributes significantly to wholebody fatty acid uptake, oxidation, and the majority of insulinstimulated glucose uptake. Eden, a wellknown pediatrician and authority on childhood nutrition and obesity together with expertly. Diet induced obese dio mouse models c57bl6ntac male. Obesity is a noncommunicable, multifactorial disorder characterized by relentless differentiation and accumulation of white adipose tissue wat culminating in comorbidities such as type 2 diabetes and cardiovascular disorders cvds.
Specific insulin sensitivity and leptin responses to a. E2f8 promotes hepatic steatosis through fabp3 expression in. International database resource for the laboratory mouse, providing integrated genetic, genomic, and biological data. The jackson laboratory maintains an inventory of dietinduced obesity dio models, and can place any strain on any fully customized diet so that your mice are ready when you need them. Upregulation of inos has been clearly implicated in obesity and diabetes 10,11, and no overproduction induced by highfat diet was reported to downregulate the insulin signaling in.
The prevalence is increasing rapidly in numerous industrialized nations worldwide. Ingested cocoa can prevent highfat dietinduced obesity by regulating the expression of genes for fatty acid metabolism naoko matsui a, ryoichi ito a, eisaku nishimura, ph. Childhood obesity and the zebrafish as a model for the. In 2010, oka and collaborators proposed a dietinduced obesity dio protocol by a diet administered over a short time in adult zebrafish, and in 2011, other groups proposed a test to evaluate obesity in larvae. An international study has revealed a striking geneticenvironmental interaction. Although zebrafish larvae have been used in genetic and chemical screening experiments to identify novel genes involved in the regulation of energy homeostasis and potential therapeutic targets to treat obesity,23, it remains unclear whether zebrafish can be used as a model for dietinduced obesity dio, similar to that observed in mammals.
Oct 21, 2010 obesity is a multifactorial disorder influenced by genetic and environmental factors. The purpose of this study was to establish a zebrafish model of dietinduced obesity dio. Consequently, zebrafish might serve as a good animal model for research into obesity induced by highfat diets. Diet induced obesity in zebrafish shares common pathophysiological pathways with mammalian obesity. Edens anecdotal examples of obese patients and their families which offers useful new information on obesity. You can select a topic to find the latest site content relating to that specialty, including links to other relevant journals and organizations that you may find interesting. In order to reduce the risk factors listed, healthcare providers, parents, and children can develop meal and exercise plans in order to maintain a healthier lifestyle.
Overfeeding and a highfat hf diet induce body fat accumulation in zebrafish through pathophysiological pathways common with those underlying mammalian obesity. New research suggests there may be a biological reason why fatty and cholesterolrich foods are so appealing together. We have characterised molecular adaptation to high fat diet hfd feeding in inbred mouse strains widely used in genetic and physiological studies. Nongenetic inheritance of dietinduced obesity in mice. Thirst is an early symptom of hyperglycemia, as excess nonreabsorbed glucose is. Obesity but not high fat diet results in lymphatic dysfunction gabriela d. Current study comprises usage of a diet induced obesity model by proposing high fat diet on adult female rats and examination of the livers of these obese rats under light microscope. Taconic biosciences maintains an inventory of c57bl6ntac male mice on 60% kcal high fat irradiated diet. Obesity prevention for children reflects 40 years of clinical practice by an unusually astute, highly competent, and compassionate clinician with a particular interest in nutrition and obesity. Diet induced obesity suppresses the secretory capacity of the mgs. Obesity is a multifactorial disorder influenced by genetic and environmental factors. Impact of physician bmi on obesity care and beliefs bleich.
Typically mice, rats, dogs, or nonhuman primates are used in these models. The nature and problem of obesity the pressing need for criteria for evaluating weightloss programs stems from the scope of the problem of obesity in this country and the evidence that it is worsening, in spite of extensive individual and programmatic efforts to achieve weight control. A zebrafish model of dietinduced obesity dio has been created by overfeeding with a high fat diet of artemia oka et al. Zebrafish possess many structural and functional similarities with humans and have been used to model various human diseases, including a genetic model of obesity. Obesityinduced inflammation is associated with alterations.
Hf and hfc dietsinduced hepatic steatosis in zebrafish larvae. While advanced stage nonalcoholic hepatic steatosis and steatohepatitis nash result ultimately in fibrosis and cirrhosis, the molecular basis for lipid droplet formation is poorly understood. Nov 14, 2015 hf and hfc dietsinduced hepatic steatosis in zebrafish larvae. Zeigler zebrafish diet, 1lb bag during this covid19 pandemic, pentair global operations and supply teams are working diligently to help ensure our valued customers are getting the best possible service and delivery during this time. Nutrigenomics of high fat diet induced obesity in mice. Nicotine plus a highfat diet leads to intramyocellular fat deposition in a mouse model of diet induced obesity cigarette smoking has direct a cigarette smoking has direct adverse effects on muscle function.
Diet induced adipocyte number increase in adult rats. Highfat dietinduced obesity impairs insulin signaling in. In this study, we tested the effects of high fat hf diet on metabolism and skeletal acquisition in young, rapidly growing female mice from two strains. Diet induced obese dio mouse models c57bl6ntac male mice. It is intended to mimic the most common cause of obesity in humans. Magnolia extract bl153 ameliorates kidney damage in a high fat diet induced obesity mouse model wenpengcui, 1,2 yangweiwang, 1,2 qiangchen, 2,3 weixiasun, 2,4 lucai, 2 yitan, 2.
We carried out physiological tests, plasma lipid assays, obesity measures, liver. The present study aimed to investigate whether the inclusion of three fatty fish servings per week within a hypocaloric diet may have specific healthy effects on insulin and leptin functions. Thus, we tried to understand the effects of obesity on adult female liver. The zebrafish as a model to study intestinal inflammation. Research article a novel wistar rat model of obesityrelated. Zebrafish research shows how dietary fat regulates.
Fvb, which resists diet induced obesity, and b6, which readily develops diet induced obesity hu et al. Fvb, which resists dietinduced obesity, and b6, which readily develops dietinduced obesity hu et al. Obesity is shown in a mouse model of liver cancer to strongly enhance tumorigenesis. High fat dietinduced obesity modifies the methylation. Dietinduced obesity models diets high in fat have been attributed to a host of diseases, including obesity, metabolic and inflammatory diseases. Obesityinduced gut microbial metabolite promotes liver.
Consumption of cholesterol rich diet and lack of physical activity in coordination with sterol regulatory element binding proteins srebps, leptin and ghrelin. Our results suggest that a diet rich in fat, instead of protein or carbohydrate, induced body fat accumulation in zebrafish with mechanisms that might be similar to those in mammals. This page brings together all our content articles, publications and videos on each of the 10 key nutrition topics. Importantly, not only is the zebrafish model useful in the study into underlying pathways of human disease, it can also serve as a great model to study foodinduced intestinal inflammation in aquaculture fish species such as atlantic salmon. Since rats and mice show a similar relationship, they are considered an appropriate model. High fat plus high cholesterol diet lead to hepatic. The other group of zebrafish was fed with artemia sufficient to meet their energy requirements 5 mg dry weightdayfish. Larvae fed with control diet rarely developed steatosis after 7 and 10 days feeding 0 % and 11 %, respectively. Obesity is an epidemic problem in the world and is associated with several health problems, including diabetes, cardiovascular disease, respiratory failure, muscle weakness, and cancer. Pdf dietinduced obesity in zebrafish shares common. There are many risk factors for childhood obesity which include poor diet, low physical activity, genetics, eating to cope with emotions, and fast food mayo clinic, 2014.
Research article a novel wistar rat model of obesityrelated nonalcoholic fatty liver disease induced by sucroserich diet marialuizar. High fat plus high cholesterol diet lead to hepatic steatosis. Highfat diet impairs cognitive function of zebrafish. The diet induced obesity model dio model is an animal model used to study obesity using animals that have obesity caused by being fed highfat or highdensity diets. The precise molecular mechanisms by which obesity induces these health problems are not yet clear. Taconic uses research diets industry standard high fat rodent diet, d12492. Feeding hcd for 8 weeks resulted in exogenous obesity confirmed by higher body mass index bmi values of obese males 0. Common pathways underlie the pathology of mammalian. Nutritional factors play important roles in the etiology of obesity, type 2 diabetes mellitus and their complications through genotype x environment interactions. The aim of this study was to examine the anti obesity effects of boiled tuna extract in c57bl6n mice with obesity induced by a highfat diet hfd. Jun 26, 20 obesity is shown in a mouse model of liver cancer to strongly enhance tumorigenesis. International database resource for the laboratory mouse, providing integrated.
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